Histochemical correlates of -glucuronidase and sodium ion transport

Journal/Book: [From the Proceedings of the Physiological Society 7-8 July 1967] Journal of Physiology 192 13-14P. 1967;

Abstract: By BRYAN BALLANTYNE Department of Anatomy School of Medicine University of Leeds Biochemical and histochemical studies of the avian supra-orbital gland have demonstrated increased -glucuronidase activity associated with the transport of Na+ (Ballantyne & Wood 1967). The histochemical distribution of this enzyme has therefore been determined in several tissues known to transport Na+ as a part of their physiological function. Fresh frozen sections of tissue were incubated in a solution containing 6-bromo-2-naphthyl--D-glucuronide as substrate and the sites of activity demonstrated by post-coupling free naphthol with o-dianisidine (Seligman Tsou Rutenberg & Cohen 1954); 25 x 106 M saccharo-1 4-lactone was used as an inhibitor for control sections. In the cortex of the mammalian kidney -glucuronidase is absent from glomeruli and present only in restricted segments of the tubules; in the medulla the enzyme occurs in the thick segments of Henle's loops; and in the papilla the collecting ducts give a positive reaction (Fig. 1 (a)). In kidney from rabbit and guinea-pig subjective assessment of the reaction suggests that more enzyme is present in the medulla and papilla than in the cortex; in the rat the reaction is strong in the cortex and medulla but weak in the papilla. In gall bladder (rabbits and guinea-pigs) the enzyme is present in high concentration only in the epithelium (Fig. 1 (b)). ... schö

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