Semi-automatic Transfer of Volatile Liquids to Paper Chromatograms |
Journal/Book: Reprinted from Nature Vol. 204 No. 4957 p.485 only October 31 1964. 1964;
Abstract: RICHARD E. BAILEY HELEN P. PIETERS JAMES H. BECK Division of Diabetes and Metabolism Department of Medicine University of Oregon Medical School Portland. 1 Clarke E. G. C. and Sowter S. A. Nature 202 795 (1964). THE recent publication of a device for applying liquids to paper chromatograms1 prompts this report of a simple arrangement for the reproducible transfer of small volumes which is in routine use in this laboratory for the application of steroid standards to paper chromatograms. It consists of a lambda pipette clamped in the vertical position with the pipette tip firmly against the surface of the chromatography paper which is supported on a perforated metal disk. A stream of compressed air for drying passes through holes in the metal disk against the lower surface of the paper (Fig. 1). In practice the pipette is filled with solvent and positioned while keeping a finger over the upper orifice to prevent leakage. Gradual transfer of solvent to paper begins when the finger is released. A Medtec Spotting Device was employed but a simpler device for support of the paper and delivery of a gas stream would serve the purpose. Experiments were performed with a variety of solvents (100-500 l. volume) containing Sudan III as indicator together with Whatman No. 2 chromatography paper and compressed air stream ... schö
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