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October 2022

The Enzymic Hydrolysis of Glutathione Thioesters1

Journal/Book: Reprinted from Anchives of Biochemistry and Biophysics Volume 95 No. 2 November 1961 S.323-328 Copyright (c) 1961 by Academic Press Inc. Printed in U.S.A.. 1961;

Abstract: From the Departments of Pharmacology University of British Columbia Vancouver Canada; and Western Reserve University Cleveland Ohio The enzyme-catalyzed hydrolysis of S-lactoyl- S-acetyl- S-acetoacetyl- S-(-hydroxy--methylglutaryl)- and S-succinylglutathione has been examined in beef liver. Purification studies have revealed that a single enzyme is involced namely glyoxalase II. A reversible non-enzymic transfer of the acetoacetyl moiety of acetoacetyl-coenzyme A and acetoacetyl-pantetheine to glutathione was found to occur at alkaline pH. . . .

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