An Apparent Difference in the Renal Clearance of CaIcium-45 and Calcium-40 in Rats

Journal/Book: Reprinted from Nature Vol. 188 No. 4750 pp. 592-593 November 12 1960. 1960;

Abstract: ROBERT C. LIKINS AND DORIS L. CRAVEN Laboratory of Biochemistry National Institute of Dental Research National Institutes of Health Bethesda Maryland. 1 Likins R. C. Posner A. S. Kunde M. L. and Craven D. L. Arch Biochem. Biophys. 83 472 (1959). IN investigating the comparative excretion of calcium-45 and strontium-89 in rats we have encountered evidence of unexpected variability in the renal clearance of calcium-45 and calcium-40. This finding may be significant in metabolic work employing radiocalcium as a tracer. Weanling female Sprague-Dawley rats (group I) were injected intraperitoneally with 1 ? 0 ml. of a solution containing carrier-free strontium-89 calcium-45 and 0?05 mgm. of calcium. Using methods previously described1 urine was collected at the end of 30 min. and 60 min. and analysed for strontium-89 calcium-45 and total calcium. One hour after injection blood was obtained from each rat by cardiac aspiration and the concentration of strontium-89 and calcium-45 in the plasma was determined. A comparable group of rats (group II) was denied food for 12 hr. and the experiment repeated. The results are presented in Tables 1 and z. Calcium-45 and strontium-... schö

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