THE PERSISTENCE IN THE BLOOD OF THE RADIOACTIVE LABEL OF ALBUMINS GAMMA GLOBULINS AND GLOBULINS OF INTERMEDIATE MOBILITY III. Comparison of Die-away Plots Following Oral and Intravenous Administration of the S35 Label to the Same Subjects

Journal/Book: Reprinted from THE JOURNAL OF LABORATORY AND CLINICAL MEDICINE St. Louis Vol. 46 No. 6 Pages 857-870 December 1955 (Printed in the U. S. A.). 1955;

Abstract: S. H. ARMSTRONG JR. M.D. DAVID BRONSKY M.D. and JEROME HERSHMAN M.S. Chicago III. From the Hektoen Institute of Medical Research of the Cook County Hospital and the Department of Medicine of the University of Illinois College of Medicine SUMMARY In further investigation of the discrepancies in apparent turnover times when estimated by in vitro-labeled I131 labeled Protein fractions injected intravenously and when estimated following autosynthesis after oral S35-labeled yeast two subjects were given enough oral yeast so labeled to permit radioactivity "die-away" plots of their autosynthesized Plasma proteins not only in themselves but following intravenous Infusion of their Plasmas into two other subjects. Plasma Proteins so infused were (in contrast to I131 labeling) "in vivo" labeled and were subjected to no in vitro fractionation prior to infusion. Of the two donors one was without known nutritional or metabolic abnormality; the other was a chronic uremic Chosen because of unusually prolonged apparent turnover times following S35 ingestion previously noted in this state. From each donor plasma was infused into a comparable pair of recipients a "normal" and a chronic uremic. With minor exceptions the results for albumins gamma globulins fibrinogen and a crude alpha1 alpha2 beta mixture exhibited the following pattern (a) apparent turnover times in the recipients following Infusion shorter than both those in corresponding donors and (when available) than those estimated in the same subjects following oral isotope administration; (b) apparent turnover times for both uremic donors and recipients longer than for corresponding "normals"; (c) flattening of dope of certain donor and recipient die-away Plots during the latter part of the study. The role of isotope reutilization in yielding these results is appraised in connection with autopsy tissue radioactivity findings in two subjects who had received oral S35. Note is made of the identity of apparent turnover times following in vitro-labeled I131 and in vivo-labeled S35 gamma globulin in two subjects in reaching the conclusion that the intravenous mute is preferable to the oral route in achieving interpretable turnover data an plasma proteins. ___MH

Zurück | Weiter