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August 2015

Biochemical and biological evidence of the activity of high potencies

Journal/Book: British Homoeopathic Journal. 1954; 44 (1): 199-251.

Abstract: 1. A method described for investigating the possilbe action of microdoses of mercuric chloride on the hydrolysis of soluble starch with malt diastase. 2. The microdoses of the mercuric chloride used in the latest crucial series carried out in 1946, 1948, and 1952, were what are termed "high potencies" made in accordance with the pharmaceutical method of preparation of drugs ordinarily used in the practice of homeotherapy.3. These microdoses were prepared by separate stages of dilution, the solution at each stage being subjected to mechanical shock. The solutions were, theoretically, "dilutions" of the order of 1 in 10-61 and on present physical theory wouId not contain any molecules of the original mercuric chloride.4. The difference in rate of hydrolysis between flask containing starch, diastase, and distilled water (controls) and flasks containing starch, diastase and microdoses of mercuric chloride (tests) were compared colorimetrically by the Spekker absorptiometer, and the frequencies of the differences statistically analysed, as the results obtain showed biological scatter. More then 500 such comparisons were carried out. The differences of means were examined by Fisjer "t" test, the variances tested and Cochrane and Cox´s test "applied where indicated. All the series gave a highly significant difference in the rate of hydrolysis between controls and tests, the microdoses stimulating the process. Statistically the significance is shown by the fact that a probability of <0-001 was obtained independently in each of the three years 1946, 1948 and 1952. The control results gave an approximately normal distribution.5. The distribution, control methods, and accessory control procedures were consired to exclude, as a cause of the effects, absorption of the original drug and the presence of extraneous contaminants by chance slely in test flasks. the only difference between control and microdose flasks was the addition of microdose, the distilled water being common to both controls and tests.6. lt was concluded that a factor, unidentified, derived from the mercuric chloride used, was present in solutions prepared by serial dilution with mechanical shock which could affect the distilled water diluent, that this cliaiige was transferable to subsequent "ultra-molecular" stages of "dilution,' and that this factor was the source of the activity in the microdose solutions producing the acceleration of the rate of hydrolysis.7. In an addendum there is described recent biological work which is also providing evidence of the presence of an active selective factor in „high potencies“ derived from Strophanthus sarmentosus by the same methods of dilution with mechanical shock.

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