Biol Pharm Bull. 2001 Nov; 24(11): 1282-5.

Cancer chemopreventive activity of odorine and odorinol from Aglaia odorata.

Inad A, Nishino H, Kuchide M, Takayasu J, Mukainaka T, Nobukuni Y, Okuda M, Tokuda H.

Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan. inada@pharm.setsunan.ac.jp

In the course of our continuing search for novel cancer chemo-preventive agents from natural sources, we have carried out a primary screening in vitro assay of the compounds isolated from Aglaia odorata. Consequently, aminopyrrolidine-diamides, odorine and odorinol, were obtained as active constituents. These compounds exhibited potent anti-carcinogenic effects in a two-stage carcinogenesis test of mouse skin induced by 7,12-dimethylbenz[a]anthracene (DMBA) as an initiator and 12-O-tetradecanoylphorbol-13-acetate (TPA) as a promoter. Further, both compounds showed remarkable inhibitory effects in two-stage mouse skin carcinogenesis models induced by nitric oxide (NO) donors such as (+/-)-(E)-methyl-2-[(E)-hydroxyimino]-5-nitro-6-methoxy-3-hexenamide (NOR-1) or peroxynitrite as an initiator and TPA as a promoter. From these results, it was concluded that odorine and odorinol inhibited both the initiation and promotion stages of two-stage skin carcinogenesis.