Hyperthermia-Induced Priming Effect in Neutrophile Granulocytes

Author(s): ELsner, J., Michalsen, A., Melchart, D., Völker, K., Dobos, G.

Abstract: Background and Objective:Whole-body infrared-A irradiaton WBIAI) according to Ardenne is a newly developed version of hypertherm. In clinical use benefits for patients with chronic infections have been reported. In order to find out more about the immunological background of the method we studied the question whether hyperthermia leads to priming effects in human polymorphonuclear leukocytes (PMN).Material and Methods:We therefore investigated the production of reactive oxygen radical species (ROS) measured by lucigenin-dependent chemiluminescence after stimulation with N-formylpeptide (FMLP, 10-6 and 10-7 M), C5a complement (10-7 and 10-8 M) or phorbolester (PMA, 10-7 and 10-8 M), in isolated PMN of 8 volunteers undergoding a 60-min hyperthermia treatment with the WBIAI. Blood was drawn 0/60/240/510 min after the start of hyperthermia treatment. In addition, we measured blood pressure, pulse, and temperature.Results:In 5 cases a significant iricrease in ROS (p < 0.05) could be measured beginning 240 min after start of hyperthermia and further increasing until the 510-min time point. These results suggest a priming effect in PMN lasting far beyond the actual treatment period. The increase of ROS production following stimulation with FMLP, C5a or PMA was 60.4 ± 21.6, 86.0 ± 23.3, and 63.3 ± 15.9% (SEM), respectively. Moreover, in all probands the maximal ROS production in PMN was observed 510 min after the beginning of WBIAI treatment. In 3 cases no difference in ROS could be observed. There was no difference in temperature, blood pressure, and pulse between responders and nonresponders.Conclusions:Our results show a hyperthermia-dependent priming effect of ROS production in PMN, suggesting an increase in immune reaction within the observation period of 510 min. Further investigations are necessary in order to specify responders and nonresponders and to characterize the results in specific diseases and the constitutions of the patients.

Keyword(s): Hyperthermie

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