Detection of 30-base pair deletion and other mutational hot spots of LMP-1 in EBV-positiv synovial membranes of patients with rheumatoid arthritis

Journal/Book: Z Rheumatol 1998; 57 Suppl. 1: 53 (P 33). 1998;

Abstract: Robert Rössle Klinik Labor für Gentherapie; 1Institut für Pathologie Universitätsklinikum Leipzig Aims: Latent membrane protein 1 (LMP-1) is identified as an oncogene due to its ability to transform rodent fibroblasts and to render them tumorigenic in nude mice. To examine the hypothesis that EBV may play an important role in the progressive proliferation of synovial cells in patients with rheumatoid arthritis (RA) we investigated LMP-1 oncogene changes in synovial membranes (SM) of patients with RA. Material and Methods: We investigated SM from patients with RA (n = 38) synovium with other inflammatory joint disease (n = 10) and 10 SM without pathological feature (nSM). LMP-1 was identified by seminested PCR. The purified amplification products were directly sequenced. Resulting sequences were compared with wild type LMP-1 (B95.8). Results: EBV-encoded LMP-1 oncogene was detected in 13/38 SM of patients with RA 1/10 in other inflammatory synovium and 1/10 in nSM. The 30-base pair deletion was present in 4 SM from patients with RA but not in others. Only 2/13 cases showed the wild-type sequence. In all other cases LMP-1 oncogene revealed different mutations also in non rheumatoid or in normal synovial tissue. Conclusion: In SM of patients with RA EBV is present significantly higher then in patients with other inflammatory joint disease. The functional significance of LMP-1 deletions and mutations needs further investigations. le

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