Auditory cortex neurons: Primary culture and ion channel activity in rat

Author(s): Hall, D. B., Narahashi, T.

Journal/Book: Acta Oto-Laryngol. 1995; 115: PO Box 2959 Toyen, Journal Division, Customer Service, N-0608 Oslo, Norway. Scandinavian University Press. 776-782.

Abstract: We have developed a primary dissociated cell culture of the fetal (E17) and post-natal (P0-P10) rat auditory cortex. Pyramidal and non-pyramidal cells had a mean cross-sectional diameter of 12.73 +/- 1.80 mu m (mean +/- S.D., n = 25) and 17.58 +/- 1.67 mu m (mean +/- S.D., n = 10), respectively, measured at 6 days in culture. These cells were viable for as long as 18-21 days. They expressed voltage-gated sodium and potassium channel currents as early as one day in culture, and at various phases in cell culture. Sodium current, activated at membrane potentials more positive than -60 mV, displayed fast activation and inactivation kinetics. Fifty percent inactivation of sodium channels occurred at a pre-pulse potential of -63 mV. Delayed rectifier potassium channels were activated at potentials positive to -40 mV. Large hyperpolarizing constant current pulses elicited anode break action potentials, and large depolarizing constant current pulses exhibited rectification indicative of the delayed rectifying potassium channel activity.

Note: Article EJ Moore, Michigan State Univ, Dept Audiol & Speech Sci, Auditory Neurosci Lab, 378 CAS Bldg, E Lansing, MI 48824 USA

Keyword(s): cell culture; patch-clamp; sodium channel; potassium channel; ROOT GANGLION NEURONS; SODIUM CURRENTS; MEMBRANE; CELLS

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