Zur Wachstumsstimulation von Bifidusbakterien durch lösliche Ballaststoffe vom Fruktantyp

Author(s): Amanu, S.

Abstract: The growth behaviour of the Bifidus bacteria was tested with pure inulin and fructanes from the storage organ (bulb) of Topinambur (helianthus tuberosus). The initial tests comprised 10 species of this genus, which can be present in faeces. More detailed examinations were performed with bacteria, which are very often present in the intestinal flora: B. adolescentis, B. breve, B. catenulatum and B. infantis. The fructane containing substrata included Helianthus tuberosus preparations from homoeopathy as well as concentrated Topinambur compressed juice and a roughage product made of bran with a Topinambur concentrate in the form of a granular powder. The products have been subjected to a stomach/appendix digestion with an addition of pancreatin in an in vitro experiment in the environment of an artificial intestinal juice with a pH of 6.8 and the assimilable sugar has been removed. This intestinal juice has been inoculated with 106 bacteria/ml and has been examined for up to 10 days with respect to an increase in cells, a change in the pH, the consumption of substrate and the formation of L(+) lactate as well as D(-) lactate under anaerobic conditions in a CO, atmosphere. There was an increase of up to 109 bacteria/ml.The pH decreased to a value between 4.1 and 4.8. From the fructane substrate predominantly lactic acid was produced with a high portion of L(+) lactate. The catabolic reaction was exogenous, so that the not metabolized portion of the fructanes still shows a roughage character. Comparisons with carboxymethylcellulose showed a growth stimulation of this carbohydrate in the case of Bifidus bacteria. A mixture of bran and concentrated Topinambur compressed juice showed the best growth stimulation.

Keyword(s): Inulin

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