Ultraviolet Difference Spectra in Human Hemoglobin

Journal/Book: THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 245 No. 3 Issue of February 10 pp. 555-558 1970. 1969/1970;

Abstract: II. DIFFERENCE SPECTRA IN ISOLATED SUBUNITS OF HEMOGLOBIN* (Received for publication July 22 1969) From the Departments of Physiology and Medicine the Albert Einstein College of Medicine Bronx New York 10461 * This investigation was supported in part by United States Public Health Service Research Grants HE 07451 from the National Heart Institute and AM 04502 from the National Institute of Arthritis and Metabolic Diseases and in part by Research Grant G67 45 from the Life Insurance Medical Research Fund. Â‡ Career Scientist of the Health Research Council of the City of New York Contract I-232. § Career Scientist of the Health Research Council of the City of New York Contract I-392. 1 The abbreviation used is: PMB p-mercuribenzoate. SUMMARY Hemoglobins A PMB and PMB (where PMB is p-mercuribenzoate) lack the pH-dependent and form-dependent ultraviolet difference spectra seen in hemoglobin A. Hemoglobin H exhibits difference spectra which are smaller than those in hemoglobin A and in the case of pH-dependent spectra at different wave length location. A difference spectrum of reconstitution of hemoglobin. A from hemoglobins A and H exists at pH 8.5 but not at pH 6. The major perturbation in this spectrum is probably attributable to the chromophore of tyrosine C7 (42). schö

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